One of the first pieces of evidence that GnT-V is involved in melanoma progression came from the finding that hybrids produced by in vitro fusion of normal macrophages with Cloudman S91 melanoma cells display increased GnT-V activity, β1,6 branching in glycoproteins, upregulation of integrin subunits α3, α5, α6, αv, β1 and β3, and increased metastatic potential in vivo and motility in vitro [74,75]. This evidence concerns the gene MGAT5 and melanoma.