qRT-PCR analysis demonstrated that HSC2 marker genes, Acta2, Fgl2, Fhl2, and Mapf4 were robustly expressed in 2-week CCl4 treated mouse liver, but their expression dropped significantly in the mouse liver after 4-week CCl4 exposure (Figure 4B), which suggests that there is an attenuation of HSC2 population at the late stage of liver fibrosis. This evidence concerns the gene FGL2 and Hepatic fibrosis.