qRT-PCR analysis demonstrated that HSC2 marker genes, Acta2, Fgl2, Fhl2, and Mapf4 were robustly expressed in 2-week CCl4 treated mouse liver, but their expression dropped significantly in the mouse liver after 4-week CCl4 exposure (Figure 4B), which suggests that there is an attenuation of HSC2 population at the late stage of liver fibrosis. Here, ACTA2 is linked to Hepatic fibrosis.