PTEN and neoplasm: In vivo, NOD/SCID mice injected with 143B cells stably transfected for the forced overexpression of PTEN displayed reduced bone destruction, mitigated tumor burden, decreased CXCR4 mRNA and protein levels (as evaluated by quantitative PCR and immunohistochemistry in tumors harvested from mice), and reduced metastatic tumor growth in the lungs, compared to control mice [254].