In particular, somatic copy number variations of the IGF gene family occurr in about 7% of cases [60,153] while fluorescence in situ hybridization (FISH) indicated a 9.2% [153] to 14% [60] of osteosarcoma cases displaying amplification of IGF1R gene with corresponding increased IGF1R transcription and downstream activation of the PI3K/Akt/mTOR pathway [153]. This evidence concerns the gene IGF1 and osteosarcoma.