This particular study observed the significance of HIF1α in gallstone formation and biliary phenotypes within NAFLD conditions, and after 1 week of feeding a cholesterol and cholate-rich diet (CCD) in mice, 0% gallstone formation was observed in hepatocyte-specific HIF1α knockout (iH-HIFKO) mice compared with 60% gallstone formation in control mice [75]. The gene discussed is HIF1A; the disease is gallstones.