To answer this question, coronal brain sections of glioma-bearing mice treated with 3 × 108 or 3 × 109 sEVs derived from BV2 cells were analyzed for the expression of the proliferation marker Ki67 by immunofluorescence: the data reported in Figure 3C,D showed a significant reduction of Ki-67 staining upon treatment with both 3 × 108 and 3 × 109 sEVs (derived from 106 and 107 BV2), and the numbers of proliferating cells were significantly different between the two treatments. This evidence concerns the gene MKI67 and glioma.