A significant accumulation of NGB in the G2 grade of post-menopausal patients compared to the normal counterpart is reported in Figure 1A. To further confirm the high level of NGB in breast cancer tissue, paraffin-embedded sections of ERα+ invasive ductal carcinoma grade G2 from 10 breast cancer patients and their healthy counterparts were subjected to immunohistochemistry staining against NGB. This evidence concerns the gene ESR1 and invasive ductal breast carcinoma.