Therefore, we knocked out all ZC3HC1 alleles in U-2 OS cells too, which represent a hyper-triploid, chromosomally highly altered female cell line that stems from an osteosarcoma ([114]; see also characteristics for the U-2 OS [ATCC HTB-96] cell line at [115]) and is therefore of mesodermal origin as well. The gene discussed is ZC3HC1; the disease is osteosarcoma.