Although the plasma membrane (PM) is the established localization of Arf6 [35], in uninfected Balb 3T3 cells [6] and MCMV-infected cells immediately after infection (0 hpi), Arf6 was found at membranous structures dispersed through the cell cytoplasm and was not detected on the PM (Figure 3C). This evidence concerns the gene ARF6 and infection.