In conclusion, by using the label-free quantitative proteomic technology, we analyzed the content of EVs cargo isolated from cell lines bearing an IPF phenotype and identified differentially expressed proteins associated with human IPF progressions, such as TNC, IGFBP7, FBN1, COL1A1, COL1A2, and LOXL1. Here, IGFBP7 is linked to idiopathic pulmonary fibrosis.