B16F10 melanoma secreted MFGE8 (1.65 ± 0.04 ng/mL), which is similar amount of MFGE8 secreted by Mo-MDSCs and Gr-MDSCs without TGF-β (1.06 ± 0.44 ng/mL and 1.91 ± 1.23 ng/mL, respectively) where S-MFGE8 is preferentially expressed over L-MFGE8. Here, TGFB1 is linked to melanoma.