To further examine ALDH1A3 expression and cell migration abilities, we established an ALDH1A3-overexpressing KKU-M005 cell line and knocked down ALDH1A3 in the KKU-M213 cell line, which expressed the highest ALDH1A3 levels among the three cholangiocarcinoma cell lines (Figure 1D). The gene discussed is ALDH1A3; the disease is cholangiocarcinoma.