AXIN1 and colorectal carcinoma: To examine whether APC truncation participates in 5-FU resistance in CRC, we transfected APC mutants (Figure 1A) lacking EB1/DLG-binding (APC 1-2644), microtubule- and AXIN-binding (APC 1-1941), and β-Catenin binding and downregulation (APC 1-1309) domains, respectively, into HCT-116 cells that carry full-length APC.