In our study, in addition to downregulating the expression of HNF1α and SREBP2, pterostilbene also decreased the protein expression of HIF1α and Nrf2, which has not been previously investigated, implicating the role of pterostilbene as a potentially promising treatment strategy against hypercholesterolemia. The gene discussed is NFE2L2; the disease is familial hypercholesterolemia.