To assess the role of iron in NaIO3-mediated AMD-like changes in ARPE-19 cells, three independent sets of sub-confluent cultures were exposed to 10 mM NaIO3 for 24 h, and the expression of major iron modulating proteins ferritin, transferrin receptor (TfR), hepcidin, and ferroportin (Fpn) was evaluated by WB. Here, HAMP is linked to age-related macular degeneration.