In the studies in which C57Bl/6J or C57Bl/6CrJ strains have been successfully employed, the subcutaneously-implanted insulin pellets that we found are essential to prevent severe hyperglycemia and pregnancy loss in the periimplantation period [13] either were not used, or were surgically removed after implantation, so the glucotoxicity may be more severe than that to which our mice are subjected [35,36,37]. This evidence concerns the gene INS and Hyperglycemia.