In addition, the inhibitory effect of CK1D008 on clonal growth was also evaluated in HH—GLI-dependent pancreatic (PANC1), NSCLC (A549), glioma (LNT-229) and CRC (HT29, HCT15, HCT116) tumor cells [6,60,61,62]. This evidence concerns the gene GLI1 and central nervous system cancer.