GLI2 and neoplasm: To evaluate whether enforced overexpression of the GLI activator proteins GLI1 and GLI2 can attenuate the anti-tumor effect caused by CSNK1D inhibition, we overexpressed in A673 cells HA-tagged GLI1 and an active form of GLI2 [63], respectively (Figure S5G), and treated GLI1, GLI2, or empty-vector control cells with CK1D008.