Furthermore, expression of HHLA2 in ICC tissues was more common than that of PD-L1 (49.0% vs. 28.1%, respectively); overexpression of HHLA2 was associated with decreased CD3 + TIL and CD8 + TIL numbers and higher CD4+ Foxp3+/CD8 + TIL ratios, which affected OS. The gene discussed is CD8A; the disease is intrahepatic cholangiocarcinoma.