CD4 and cancer: In contrast, the control CD4+ T cells stimulated for 12 days and restimulated for an additional 3 days without cancer cells (T15) exhibited less dramatic transcriptomic changes demonstrated by upregulation of 1212 and downregulation of 1097 genes when compared to CD4+ T cells stimulated for 12 days (T12) (Figure 2B), indicating that the presence of cancer cells has a stimulatory effect on CD4+ T cell transcriptomes.