Furthermore, we also observed cytotoxic T cell (CD8 + CD3+) accumulation in the brain tumors of dLGG–10 mice (134 ± 8 cells), Lipo-DOX–2 (119 ± 14 cells), and dLGG–10 + Lipo-DOX–2 (109 ± 8 cells) compared to the tumor control group (not detected). This evidence concerns the gene CD8A and neoplasm.