Pathway enrichment analysis results clearly show a linkage of progression through the cell cycle, DNA replication, and repair to 2D cell-cultured cells analyzing KEGG, Panther, and Elsevier pathways whereas upregulated genes in 3D alginate cultured melanoma cells significantly correlate with adherens and focal junctions, axon guidance, and other Rho GTPase associated pathways, as well as insulin/insulin-like growth factor (IGF), platelet-derived growth factor (PDGF) and epidermal growth factor receptor (EGFR) signaling (Figure 2B and Table S3). This evidence concerns the gene IGF1 and melanoma.