Moreover, despite that there were no macroscopic tumors, we found an increased expression of HCC markers, including Afp, Golm1, Krt19, and Birc5, in Tg SREBP-2 mice treated with DEN compared to the DEN treatment to WT mice, as well as the proliferation marker Mki67 (Figure 3G). Here, AFP is linked to hepatocellular carcinoma.