To further establish that the increase in cholesterol in the ATH model of NASH potentiates DEN-induced tumorigenesis, we used transgenic mice expressing SREBP-2 (Tg SREBP-2), the master transcription factor that regulates cholesterol synthesis in the endoplasmic reticulum (ER). The gene discussed is SREBF2; the disease is metabolic dysfunction-associated steatohepatitis.