In line with the progressive damage, DEN potentiated hepatic fibrosis in rats fed the ATH diet compared to the CD feeding, as seen by the increase in the expression of Col1a1, Acta2, and Tgfβ, and Sirius red staining, as well as α-SMA (Figure 1D–F). The gene discussed is ACTA1; the disease is Hepatic fibrosis.