ACTA2 and Hepatic fibrosis: Of relevance, HFHC-fed mice exhibited liver fibrosis, as seen by Sirius red staining (Figure 4B), the increased expression of Acta2 and Col1a1, and the hydroxyproline levels (Figure 4D), accompanied with a higher level of the genes involved in inflammation like Tnfα and Ccl2 (Figure 4E), resulting in a higher NAS score in mice fed a HFHC diet compared to HFD-fed mice (Figure 4F).