Moreover, while no tumors were found yet in these conditions, increased mRNA levels of the HCC markers, including Afp, Golm1, Krt19, Birc5, Cd44, and Lyve1 (Figure 2H) [31,32,33,34], as well as markers of liver regeneration Mki67, Pcna, and Ccne1 (Figure 2H) and the protein levels of Gp-73 and CK-19 (Figure 2I,J), were observed in DEN-treated mice fed the ATH diet compared to DEN CD-fed group. This evidence concerns the gene AFP and hepatocellular carcinoma.