Filipin staining indicated the increase of free cholesterol levels in Tg SREBP-2 mice compared with WT mice, while Sirius red staining revealed liver fibrosis in Tg SREBP-2 mice that was potentiated by the DEN treatment (Figure 3C,D), in line with the increased expression of fibrosis genes Col1a1, Acta2, Tgfβ, and Spp1 (Figure 3E). This evidence concerns the gene TGFB1 and Hepatic fibrosis.