CD diet feeding caused macrovesicular steatosis, as revealed by Oil Red staining and characterized by an increased liver triglycerides content, while ATH administration resulted in microvesicular steatosis with the accumulation of cholesterol levels in the liver, mostly in a free form, as seen by filipin staining, that, unlike CD feeding, resulted in liver damage (H&E) and serum ALT release (Figure 1A–C). Here, GPT is linked to steatosis.