SREBF2 and Hepatic fibrosis: Filipin staining indicated the increase of free cholesterol levels in Tg SREBP-2 mice compared with WT mice, while Sirius red staining revealed liver fibrosis in Tg SREBP-2 mice that was potentiated by the DEN treatment (Figure 3C,D), in line with the increased expression of fibrosis genes Col1a1, Acta2, Tgfβ, and Spp1 (Figure 3E).