CD4 and neoplasm: Similarly, CD3+CD8+ PD-1+PD-L1+ cells were also found at a significantly higher frequency in tumour-infiltrating tissue compared with TDLNs (median 1.62 (range 0.02–34.6) vs. 0.12 (range 0–0.87) p < 0.05), similarly for CD3+CD4+TIM-3+PD-L1+ cells (median 5.03 (range 0.02–42.8) vs. 0.11 (range 0–0.41), p < 0.05) (Figure 5).