Concordant with our in silica and in vitro data, the post-study IHC staining of harvested tumor samples showed that, compared to the expression in the control samples, enzalutamide treatment, inoculation with PC3_shGSE1, or PC3_shGSE1 coupled with enzalutamide treatment, suppressed the GSE1, MDR1, and OCT3/4 protein expression levels, in increasing order of magnitude. The gene discussed is ABCB1; the disease is neoplasm.