Therefore, we speculate that TTX-S type VGSCs modulators HNTX-III and JZTX-I can affect the proliferation, metastasis and invasion of prostate cancer cells by regulating the expression and phosphorylated level of causal proteins (potential biomarkers) such as EEF2, U2AF2 and FLNC and the related processes including “energy metabolism and transition” and “cell adhesion and migration”. Here, U2AF2 is linked to prostate cancer.