Interestingly, however, DM + HFD + CKD swine showed a clockwise rotation in the relationship between eNOS protein levels and myocardial NO2− + NO3− levels as compared to Normal swine, so that at a given level of eNOS protein expression, the myocardial NO2− + NO3− levels were lower (Fig. 8a). The gene discussed is NOS3; the disease is chronic kidney disease.