Although it could be argued that the sensitivity of the Griess assay used to assess NO metabolites levels may not be sufficient to detect subtle differences in levels of NO metabolites [51], it should be noted that the ratio between p-VASP and VASP was also not different between the two groups, further suggesting that NO-cGMP signalling was maintained in bulk myocardial samples from DM + HFD + CKD swine. Here, VASP is linked to chronic kidney disease.