NOS3 and chronic kidney disease: mRNA and protein levels of eNOS, eNOS phosphorylation at the stimulatory Ser1177 site, eNOS glutathionylation and eNOS monomer to dimer ratio were all maintained, while eNOS phosphorylation at the inhibitory Thr495 site was even slightly lower in DM + HFD + CKD swine (Fig. 7a–f).