To clarify the role of P4HA2 in cell invasion and migration, which are the most essential aspects of glioma malignancy, Matrigel invasion assay was performed to reveal that P4HA2 knockdown significantly decreased the number of invading cells in both U251 and U87MG cells at 24 hours after serum-free culture (Figure 2(c), p < 0.001). Here, P4HA2 is linked to central nervous system cancer.