SIRT7 and neoplasm: To that end, we generated tumor xenografts by implanting 4T1 cells that were stably transfected with Sirt7 shRNA (KD7) or scramble shRNA (Scram) in female Balb/c mice subjected to IF for 48 h starting on day 16 and day 22, respectively; otherwise, all animals were provided with food ad libitum.