Further subgroup analysis of various clinicopathological features of HNSCC patients from the TCGA database revealed that in subgroup analyses based on gender, age, race, disease stage, tumor grade, human papillomavirus infection, and nodal metastasis status, the transcription level of DFNA5 in HNSCC patients was consistently significantly higher than that in healthy subjects (Sup Fig. 1). Here, GSDME is linked to head and neck squamous cell carcinoma.