LMNA and Emery-Dreifuss muscular dystrophy: To identify whether other LMNA mutations have the same effect on cofilin-1 expression through pERK1/2 activation, we transiently transfected C2C12 cells with plasmids encoding lamin A variants found in EDMD (i.e., E358K, L271P, and N456I), which caused pERK1/2 activation (Figure 3F).