Analysis of the CLS cohorts of colorectal, head and neck, and breast cancers identifies a significant increase in PDL1 expression via immunohistochemistry (q = 1.03 × 10−9), as well as increases in HAVCR2 (q = 0.0077), LAG3 (q = 7 × 10−4), and PDL2 (q = 0.0357) RNA expression in colorectal cancer in elderly patients (Figure 2B) and a significant increase in LAG3 expression (q = 0.0112) in patients with HPV-negative head and neck cancer (Figure 2C), while no significant changes in immune checkpoint gene expression were identified in the breast cancer cohort (Figure 2D). This evidence concerns the gene CD274 and colorectal cancer.