Our previous study has demonstrated that IL-27 receptor-deficient (WSX-1−/−) mice were defective in the clearance of C. difficile, and they displayed increased disease severity and higher mortality compared to WT mice.16 Having observed that stimulatory effects of IL-27 on production of LL-37, along with decreased levels of CRAMP (mouse homolog for human LL-37) in WSX-1−/− mice during CDI, we next examined whether injection of exogenous CRAMP peptide into ileal loops may reduce mortality and morbidity in WSX-1−/− mice after CDI. This evidence concerns the gene IL27 and clostridium difficile infection.