DIAPH2 and anaplastic astrocytoma: In detail, one patient collected tumor specimens in all stages of MT in the present cohort.PRKCQ,PPIF,NRP1,MEFV,GGT1,FAN1, andBTKmutations were found in both DA and anaplastic astrocytoma, whereas mutations ofTBC1D19,ESRRA,DIAPH2,COG6, andCBWD3occurred in HGA.