In detail, one patient collected tumor specimens in all stages of MT in the present cohort.PRKCQ,PPIF,NRP1,MEFV,GGT1,FAN1, andBTKmutations were found in both DA and anaplastic astrocytoma, whereas mutations ofTBC1D19,ESRRA,DIAPH2,COG6, andCBWD3occurred in HGA. Here, NRP1 is linked to anaplastic astrocytoma.