ESR1 and melanoma: These data are in contrast to what we observed in ER-negative cell lines, where 27HC was shown to inhibit the proliferation of the 4T1, MDAMB436, Py230, MDAMB231, and HCC1954 breast cancer cell lines (Fig. 1c) and the BPD6 and B16F10 cellular models of melanoma (Supplementary Fig. 1a) in regular media containing lipids.