To probe the connection between GSDME-mediated tumor suppression and immune response, NSG mice lacking mature lymphocytes and perforin-deficient BALB/c mice were separately employed by the group to reveal that the antitumor effect of GSDME was both lymphocyte- and perforin-dependent and implicated the involvement of NK and CD8+ T cells. The gene discussed is CD8A; the disease is neoplasm.