GzmB was likewise shown to rapidly cleave GSDMB and activate caspase-3 in Luc-Raji and NALM-6 cells, although its release and potential to induce mouse B16 melanoma cell pyroptosis was suggested to be dependent on CAR-T cell tumor antigen affinity and co-signaling domains or its quantity when released, respectively. Here, CASP3 is linked to melanoma.