In human cancer cell lines endogenously expressing GSDMB, specifically OE19 (esophageal carcinoma), SW837 (CRC), and SKCO1 (CRC), it was further shown that GzmA delivery through electroporation or perforin was sufficient to induce GSDMB-mediated pyroptosis [35]. The gene discussed is PRF1; the disease is colorectal carcinoma.