We applied an established protocol for in vivo human osteoblast isolation [19] to obtain the alkaline phosphatase (ALPL)high/CD45/34/31low cells from the femur head-derived bone tissue of one human subject (31-year-old Chinese male) with osteoarthritis and osteopenia through fluorescence-activated cell sorting (FACS). The gene discussed is PTPRC; the disease is Osteopenia.