Comparing with the adjacent nontumor tissues, ASPM mRNA levels were significantly higher in HBV‐HCC tumor tissues with large tumor size (>5 cm; P < 0.001), high alphafetoprotein (AFP) level (≥20 ng·mL−1, P < 0.00001), slight (P = 0.026) and middle cirrhosis (P = 0.008), vascular invasion (P < 0.001), tumor embolus (P = 0.004), and low (P = 0.022) and middle differentiation (P = 0.004; Fig. 2A–F). This evidence concerns the gene AFP and hepatocellular carcinoma.