MAPT and tauopathy: In developing a high-content image-based assay in a human neuronal cell model of tauopathy suitable for screening purposes, we needed to accomplish two objectives: (1) large-scale rapid generation of reproducible (low variability) differentiated neuronal cultures from healthy and patient-derived iPSC that express physiological or pathology-relevant endogenous levels of tau, respectively, and (2) develop an image analysis pipeline that can quantitatively measure tauopathy-relevant phenotypes in a morphologically complex neuronal culture system.