Immunofluorescent staining showed that 3 h of treatment by 100 nM CBG markedly increased the number of SW480, A549 and HepG2 cancer cells with strongly stained nuclear 53BP1 foci (Fig. 2E, Additional file 2: Figure S2C), while no change in 53BP1 staining signal was evident in similarly treated NCM460, BEAS-2B and L-O2 noncancerous cells (Fig. 2E, Additional file 2: Figure S2C). Here, TP53BP1 is linked to cancer.