Our study demonstrated that 5-BDBD can prevent the decrease in the mean fluorescence intensity of MBP in the peri-hematoma region after ICH days 3 and 7 (Figs.6A, B and6F, G), showing that the P2X4R inhibitor 5-BDBD attenuated WMI induced by ICH. This evidence concerns the gene P2RX4 and hematoma.