The sensitivities were 49.1%, 93%, 92.3% with specificity of 100%, 90.9%, 71.4% respectively using the cut-off value of 155.96 ng/l for IL-33, 30.55 U/l for ADA, 25.35 pg/ml for T-SPOT.TB respectively (Fig. 3 and Table 3). The gene discussed is ADA; the disease is tuberculosis.