ADA and infection: Indeed, there was no detectable infection (based on intracellular Gag p24) of MDMs using WITO T/F virus (multiplicity of infection (MOI) of 5), whereas infection with a cell-free WT NL 4-3 ADA virus (M-tropic, MOI of 2) resulted in up to 5% of p24+ cells (Fig. S3A).