Also, multiple sequence alignments of human P2RX2 protein by ConSeq web server (PolyPhen, SIFT, and Mutation Taster) proved high conservation of this amino acid among various types of species; hence, this mutation can affect the ATP-mediated regulation activity of P2RX2 and can cause developmental abnormalities,  leading to autosomal dominant deafness. This evidence concerns the gene P2RX2 and autosomal dominant nonsyndromic hearing loss.