The following circulating biomarkers were higher in T2D developers: ketone bodies (β-hydroxybutyrate, acetoacetate and acetone), ALP, ALT, AST, creatinine, hsCRP, FPG, ferritin, GGT, GlycA, H2P, hemoglobin, hematocrit, insulin, BCAAs (isoleucine, leucine, valine), total cholesterol, triglycerides, TMAO, transferrin, and urea. The gene discussed is INS; the disease is type 2 diabetes mellitus.