Automated algorithms have been widely applied for the recognition of nuclei that can be used for segmentation of specific cells or tissue compartments, i.e., distinguishing between epithelial and stromal cells or between benign and malignant (42, 43, 44, 45, 46), detection of immune cells (47, 48), classification or quantification of certain cell states, such as mitotic cells (49), HER2 positive tumor cells in breast cancer (50), or Ki67 positive proliferative cells (51, 52, 53, 54). This evidence concerns the gene MKI67 and breast cancer.