D2HG quantitation via ELISA also showed that 0905 and TB096 cell lines produced higher levels of D2HG, whereas BT142 more closely resembled IDH1/2wt glioma cells (Fig. S2b), consistent with prior studies indicating that both IDH1wt and IDH1mut alleles are required for prominent D2HG production [8, 22–24]. This evidence concerns the gene IDH1 and glioma.