Moreover, the multiplex staining results showed that after being coplanted with RAW‐264.7‐OPN in vivo, the PD‐L1 level in NSCLC cells A549 increased significantly (Figure 6(c)) than the A549/RAW‐264.7‐Ctl cocultured group (Figure 6(d)). The gene discussed is SPP1; the disease is non-small cell lung carcinoma.