Step2: By conjointly analyzing the differentially expressed metabolic rate‐limiting enzymes from RNA‐seq data of HCC animal model and TCGA data, 12 transcripts were overlapped (RRM1, SQLE, PCK1, PYGB, G6PD, PLAT, ACSL1, RRM2, UCK2, ASS1, FBP1 and IMPDH1). The gene discussed is ASS1; the disease is hepatocellular carcinoma.