As shown in Fig. 6C and D, the puncta intensity and area of STX1A and SNAP25 in PFC neurons (NeuN+) were markedly reduced in Alzheimer’s disease patients [Intensity, STX1A, t(16) = 4.7, P < 0.001; SNAP25, t(16) = 6.0, P < 0.001; Area, STX1A, t(16) = 4.9, P < 0.001; SNAP25, t(16) = 4.9, P < 0.001, t-test], while VAMP2 puncta intensity and area were not significantly changed [Intensity, t(16) = 0.6, P = 0.54; Area, t(16) = 0.8, P = 0.40, t-test]. Here, VAMP2 is linked to early-onset autosomal dominant Alzheimer disease.