As shown in Figure 3E, compared with the sham group, the nerve cells in the MCAO/R and the vehicle-treated MCAO/R group presented significant pyknosis, broken or even disappeared or nuclear, and the brain tissue section presented severe brain injuries; after treatment with CTRP1, the nuclei were more complete, the number of nerve cells with nuclear pyknosis or broken was significantly reduced, and brain injuries in the brain tissue were overtly alleviated. This evidence concerns the gene C1QTNF1 and brain injury.