Therefore, this project intends to analyze and verify the function and molecular mechanism of TGF-β-induced novel breast cancer metastasis-related lncRNA TPA through overexpression and RNA interference, Western blot, RNA pull down experiment, and mass spectrometry identification, aiming to clarify the role and specific mechanism of lncRNA TPA in breast cancer EMT, invasion and metastasis. This evidence concerns the gene TGFB1 and breast cancer.